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The sperm cation channel (CatSper) is a specific calcium ion channel located on the membrane of sperm cells. CatSper4 acts as the core sub-unit of this channel. Its main function is to regulate calcium ions flow and sperm hyperactivation. In this study， the molecular characteristics of CDS region of sheep CatSper4 gene were studied by bioscientific analysis and systematic evolution tree was constructed. The expression of the CatSper4 gene and protein in the testes of sheep at different stages of development (2，6 and 12 months of age) was measured using real-time fluorescence measurement (qRT-PCR) and protein immunosignature (Western-blot). The results showed that the CDS region of sheep CatSper4 gene was 1341 bp long， encoding 460 amino acids. The theoretical isoelectric point (pi) of the protein is 10.85， which is an unstable hydrophilic protein with transmembrane region， no signal peptide sequence and 61 phosphorylation sites.Both the secondary structure and the tertiary structure are composed of alpha-helix， irregular curvature and β-folding and may be related toCatSper1、CatSper2、CatSper3、CatSperB、CatSperD、CatSperG、CatSperE、TMEM262、TMEM249And EFCAB9 protein. The similarity of CDS region of sheep CatSper4 gene to goat， cattle and deer was higher than 96%. The similarity with a domestic donkey was lower at 85.23%.The expression of CatSper4 gene and protein in testis of sheep at three developmental stages increased with the growth of age， and the difference of expression was significant (P<0.01). Therefore， the expression of CatSper4 in different developmental stages of sheep testis is different， indicating that it plays an important role in the regulation of spermatogenesis. The results of this study provide important scientific basis and theoretical reference for further study on the function and mechanism of male reproduction in CatSper4 sheep.
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Basic Information:
China Classification Code:S826
Citation Information:
[1]ZHANG Xiaoyi,WANG Meijie,LIU Xingwang ,et al.Analysis of the Molecular Characteristics of the CDS Region of the Sheep CatSper4 Gene and its Expression in the Testes at Different Stages[J].Journal of Economic Animal().
Fund Information:
辽宁省教育厅基础研究项目（LSNQN202005); 辽宁省科技厅博士科研启动基金计划项目（2021-BS-137）
2026-07-02
2026-07-02
2026-07-02