ZHANG Xinyu;SHI Hongyu;ZHANG Tietao;LIU Zongyue;
Genetic breeding research serves as a crucial support for the stable development of the fur animal farming industry. Since the rise of Mendelian genetics and population genetics, and with the continuous progress of quantitative genetics and molecular biology, fur breeding has gradually entered the "post-genomic era". During this period, techniques such as conventional breeding, marker-assisted selection, and genomic selection have been widely applied, promoting fur animal breeding and yielding numerous achievements. However, there is currently a lack of a systematic review of the breeding achievements and progress in fur animals, making it impossible to clearly present a referable breeding development path for the farming industry. Focusing on this, this paper introduces the current situation of the domestic and international fur animal industry and the status of variety cultivation, and elaborates on the research progress of conventional breeding, marker-assisted selection, and genomic selection techniques in the improvement of fur animal production performance, aiming to provide valuable references for subsequent fur animal research and actual production activities.
GAO Jinwei;ZHANG Rui;JIANG Qingqing;ZHANG Dan;JIA Xuying;DOU Yong;SHAO Peng;XIA Sudong;ZHOU Wenli;
To develop the short-term cryopreservation protocol for Chlorella vulgaris, optimize the associated conditions to ensure cell viability and provide high-quality biological feed for aquatic economic animals. The study used Chlorella vulgaris as the test strain, glycerol as the cryoprotectant, and the major performance indicator was the algal cell survival rate. After an initial one-factor test to establish the optimal range for short-term cryopreservation temperature, cell density, and duration, a response surface methodology was employed to refine these parameters. A one-factor experiment revealed that the temperature range of -20 ℃ to -196 ℃, the algal cell density of 2.5×10~(5) to 5×10~(5) cells/mL, and the length of 12 to 144 h were the comparatively ideal conditions for the low-temperature preservation of Chlorella vulgaris. Response surface analysis indicated that, in order of significance, preservation period, preservation temperature, and algal cell density all affect the survival rate of algal cells. Cell activity was significantly influenced by preservation duration, temperature, and cell density. The optimal conditions were determined using response surface methods to be 70 h, -78.91℃, with a cell density of 3.77×10~(5) cells/mL. A cell density of 3.77×10~(5) cells/mL, a preservation period of 70 h, and a temperature of -80 ℃ were further determined to be the optimal preservation parameters. After 24 hours, the post-thaw survival rate of algal cells was 70.11%. A technical framework for the cryopreservation of Chlorella and other freshwater microalgae is provided by these ideal conditions, which guarantee excellent cell viability after thawing.
LI Guohui;SHI Jiamin;ZHANG Huiyong;XUE Qian;ZHOU Chenghao;JIANG Yixiu;YIN Jianmei;HAN Wei;
In order to evaluate the preservation effect of the populations in National Chickens Genetic Resources (Jiangsu), three nonlinear growth models, Logistic, Gompertz and Bertalanffy, were used to fit the growth of hens in Langya chicken preservation population of two generations in 2019 and 2022. The results showed that the body weight fitting degrees (R~(2)) of three models in the two generations were all above 0.996. According to the R~(2) values, the Gompertz model had the best growth fitting effect on Langya chickens. The mature body weights fitted for the two generations ranged from 1967.965g to 1896.725g. The turning point age ranged from 6.1 to 6.3 weeks. The results were consistent with the actual situations of the preservation and production in Langya chickens. In summary,in the process of local chicken preservation, the population can be scientifically and reasonably fed and managed. And the preservation effect can be monitored based on the characteristics of the growth curve. This study will provide a scientific basis for the protection, development and utilization of local chicken genetic resources.
QU Ke;PAN Yue;ZHANG Miao;ZHAO Wanzhu;ZHEN Yuguo;SUN Zhe;WANG He;WANG Tao;
Mycotoxins cause health problems for humans and animals every year, posing significant challenges to global feed safety. In China's livestock industry, the breeding of ruminants such as cattle and sheep occupies a significant position. However, the ration used to feed these ruminants is highly susceptible to fungal invasion during the harvesting and storage stages, leading to contamination with mycotoxins. At present, research on mycotoxins contamination in feed mainly focuses on monogastric animals such as pigs and poultry, while there are relatively few reports on ruminants. This paper reviewed the harmful effects and toxic mechanism of several mycotoxins on ruminants, and discussed the detoxification methods, so as to provide reference for ruminants healthy breeding.
AN Yuting;LI Shuang;LI Anna;LI Jiacheng;MA Bo;LI Junnian;TAO Shuanglun;YANG Dongmei;
Glycoprotein is the main component of skin mucus of giant salamander, which had the functions of anti-oxidation and antibacterial. KM mice were used as research objects to verify the anti-hypoxia effect of skin mucous glycoprotein, it provides a new perspective to explore the adaptation of giant salamander to low oxygen environment in water. The mice were intraperitoneally administered with giant salamander skin mucus glycoprotein at doses of (600 mg/kg), (800 mg/kg), and (1 000 mg/kg) for 7 consecutive days, using ginsenosides as the positive control group and normal saline as the blank control group. A normobaric hypoxia experiment was conducted, followed by the measurement of cerebral edema, pulmonary edema, and the activities of nitric oxide synthase, glutathione peroxidase, superoxide dismutase, as well as the content of malondialdehyde in plasma, brain tissue, and myocardial tissue. The results showed that the skin mucous glycoprotein of giant salamander could prolong the survival time of hypoxic mice by 4.39%-15.5%, reduce the brain water content of mice by 4.16%-13.12%, and reduce the lung water content of mice by 6.90%-16.72%. The activities of total NOS, GSH-Px and SOD in plasma, brain and myocardium of hypoxic mice were significantly increased, and the level of MDA was decreased (P<0.01).The skin mucus glycoprotein of giant salamander can improve the anti-hypoxia ability of mice, which may be one of the ways for giant salamander to adapt to the low oxygen environment in water.