Journal of Economic Animal

Research Progress on Mechanism of Action and Application of Functional Feed Additives in Fur-Bearing Animal Farming

Research Progress of Breeding Technology for Fur-Bearing Animals

Production Performance Measurement and Genetic Evaluation of Fur-Bearing Animals

Analysis of Current Development Status and Optimization Suggestions for the Fur-Bearing Animal and Deer Industry in Shandong Province

Construction and Characterization of Lactobacillus Casei Lc-ST6-LFCA-TFF3 for Mucosal Repair

Salmonella is a common class of intestinal pathogenic bacteria widely present in nature， particularly within animal hosts， and represents a significant pathogen in the fields of food safety and public health. Salmonella enterica serovar Typhimurium (S. Typhimurium) is a typical serovar capable of infecting a variety of animals， including mice and humans. Although antibiotic treatment can effectively inhibit bacterial growth and alleviate symptoms， improper use may lead to drug resistance and even disrupt intestinal microecological balance. Therefore， antibiotics must be used with caution， and alternative therapeutic approaches， such as probiotic therapy， should be explored. In view of this， the present study utilized Lactobacillus casei ATCC 393 as the host probiotic strain. The ST6GALNAC1， LFCA， and TFF3 genes were fused and directionally cloned into the pPG-612 vector. The resulting recombinant plasmid was introduced into L. casei via electroporation to generate a novel functional probiotic strain， designated as Lc-pPG-ST6-LFCA-TFF3 (Lc-pPG-SLT). Cellular safety assays were conducted to evaluate the biosafety of the engineered strain towards intestinal epithelial LS174T cells.RT-qPCR analysis revealed that the engineered strain significantly upregulated the expression of tightening junction proteins Occludin and Claudin-1， while it did not induce the abnormal elevation of the leaky protein Claudin-2 typically caused by pathogenic invasion， and simultaneously suppressed the expression of inflammatory factors. The development of engineered strain provides an experimental basis and a novel strategy for the development of genetically engineered probiotics targeting intestinal barrier repair.

Establishment of a SYBR Green I-Based Real-Time PCR Method for Absolute Quantification of the Feline EGFR Gene and Analysis of Its Tissue Expression Profile

Epidermal growth factor receptor (EGFR) is a key regulator of cell proliferation， differentiation， and tissue repair， and its dysregulation is closely associated with the pathogenesis of multiple tumors. To establish a quantitative baseline of EGFR expression in normal tissues of companion animals， an absolute quantitative real-time PCR (qPCR) assay based on SYBR Green I was developed in this study. A recombinant feline EGFR plasmid was used as the standard to generate a calibration curve for copy number determination， and EGFR mRNA abundance in multiple tissues from cats and mice was subsequently compared. The assay showed high amplification specificity， with a correlation coefficient of R = 0.996， an amplification efficiency of 99.7%， a linear dynamic range of 5.2 × 10^8 to 5.2 × 10^1 copies/μL， and a detection limit of approximately 5.2 × 10^1 copies. After excluding replicate outliers that deviated substantially from the mean， EGFR expression in 13 tissues from healthy cats ranged from 1.64 × 10^2 to 2.02 × 10^3 copies/μg total RNA， with the highest level detected in the small intestine (2024.178 copies/μg total RNA)， followed by the conjunctiva (778.595) and heart (724.542)， whereas the spleen showed the lowest level (164.276). In 12 tissues from healthy mice， EGFR expression ranged from 1.97 × 10^2 to 2.38 × 10^3 copies/μg total RNA， with the highest level in the kidney (2382.941)， followed by the liver (922.530) and spleen (858.902). Cross-species comparison revealed that EGFR expression in the small intestine and conjunctiva was higher in cats than in mice， by approximately 6.67-fold and 3.32-fold， respectively， whereas expression in the kidney and liver was higher in mice， by approximately 5.70-fold and 2.59-fold， respectively. Taken together， the absolute quantitative qPCR assay established in this study provides comparable cross-tissue copy number measurements and defines a quantitative baseline for EGFR expression in normal feline and murine tissues， thereby offering useful reference data for comparative studies， biomarker discovery， and safety evaluation of EGFR-targeted therapeutics.

Progress in the Development and Application of Silage for Ruminants^*

Silage is a kind of high moisture feedstuff through anaerobic fermentation by lactic acid bacteria. The silage possess high nutritional value，excellent palatability，and ease in storage and transportation.The use of silage in ruminant production contributes to improved production efficiency by increasing both feed intake and feed conversion rate.Therefore，this paper summarizes the types of silage，the methods for evaluating its nutritional value， and its applications in animal production.This information is synthesized in order to inform and guide future endeavors in silage development and application.

Molecular Characterization of PRRSV in a Pig Farm in Heilongjiang Province

To characterize the genetic variation of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in a large-scale pig farm in Heilongjiang Province， a total of 354 samples were collected from the farm between 2021 and 2024. RT-qPCR detection revealed 38 positive samples， yielding an overall positivity rate of 10.73%. No significant upward or downward trend in annual positivity rates was observed from 2021 to 2024. Phylogenetic， homology， and amino acid variation analyses were performed on the 38 positive strains. Phylogenetic analysis based on the ORF5 gene showed that three strains were classified into sublineage 1.8， 25 into sublineage 1.5， and 10 into lineage 8. Homology analysis indicated that the nucleotide sequence identities among the 38 PRRSV strains ranged from 82.7% to 100%， and the amino acid sequence identities ranged from 81.7% to 99.5%. Amino acid mutation analysis revealed that the signal peptide， neutralizing epitope， T?cell epitope， and B?cell epitope of the GP5 protein all contained amino acid substitutions. Among these strains， 10 retained both virulence-related residues R¹³ and R¹⁵¹. Through long?term monitoring of PRRSV prevalence in a swine farm in Heilongjiang Province from 2021 to 2024， combined with molecular characterization of the positive samples， this study comprehensively elucidates the epidemic characteristics and genetic diversity of PRRSV in the farm， providing a theoretical basis for understanding the epidemiological status of PRRSV and for the prevention and control of future outbreaks.

The Application of Microecological Agents in the Production of Special Economic Animals

With the advancement of green animal husbandry and the implementation of antibiotic restriction policies， microecological agents have emerged as a pivotal alternative to antibiotics for promoting healthy animal production， attributed to their inherent advantages of safety， environmental benignity， and non-residue. This review summarizes the mechanisms of action of microecological agents and the latest research progress in their application to specialty economic animals， with a particular focus on elucidating their regulatory effects on intestinal microecological homeostasis and immunometabolism in these animals. Specifically， they exert a crucial targeted regulatory role in resisting pathogenic microbial invasion， optimizing nutrient digestion and absorption， as well as enhancing production performance and host immune competence. This review is intended to provide theoretical references for reducing antibiotic usage and improving the breeding efficiency of specialty economic animals.

A Study on the Extraction Separation and Anticancer Activity of Velvet Antler Protein

Application of Ecological Niche Models for Selection of Species Habitat

Advances on the Chemical Components and Pharmacological Activities of Velvet Antler

The Effect of the Alcohol Extract of Pilose Antler on the Red Blood Cells′ Immunologic Function in the Mice Treated by Cyclophosphamide

Pathogens of Intestinal Diseases in Giant Panda

Interpretation of Development of Chinese Pet industry in 2024

Utilization of Resources Insects

Advances on the Chemical Components and Pharmacological Activities of Velvet Antler

Research Progress of Immune Adjuvant

Advanceson Heat Shock Protein