ZENG Xuejiao;ZHAO Xilong;Monakhov V.G.;LI Bo;
To understand the sexual dimorphism of the sable skull and to identify the evidence used for sex determination based on skull characteristics, 10 indices from 50 sable skulls with known sexes were measured. Descriptive analysis and principal component analysis were conducted to explore the sexual dimorphism of the skull, followed by discriminant analysis for sex identification on samples with unknown sexes.The results indicated significant sexual dimorphism in 8 skull indices between male and female sable skulls(P<0.05), with the exception of the length and width of the tympanic bulla. According to the discrimination functions, the identification results of the single variables for profile length and basal length were consistent with the results of molecular biological sex identification, with an accuracy rate of 100%. The identification results of the nine combined variable groups—including “profile length+upper tooth row length”, “basal length+upper tooth row length”, “profile length+zygomatic width+upper tooth row length”, “profile length+basal length+zygomatic width+upper tooth row length”, “profile length+lower tooth row length”, “profile length+upper tooth row length+lower tooth row length”, “profile length+basal length+upper tooth row length+lower tooth row length”, “profile length+zygomatic width+upper tooth row length +lower tooth row length”, and “profile length+basal length+zygomatic width+upper tooth row length+lower tooth row length”—also exhibited perfect agreement (100% consistency) with the molecular-based sex identification.These findings provide a scientific foundation for sex identification methods based on cranial indices, emphasizing the utility of morphological traits in forensic and ecological studies.
ZHANG Yu;YAN Xinrui;LI Xin;HAN Xintong;LIU Chang;LIU Wei;LI Yaxin;JIA Boyin;
Arthritis is a degenerative osteoarthritis characterized by damage and loss of chondrocytes. Due to the lack of blood vessels in cartilage, it is increasingly important to find a treatment for arthritis that is difficult to selfheal. In the rapid growth period, pilose antler can grow 2 cm a day, which is mainly composed of stem cells and their differentiated cartilage, and contains a large number of blood vessels. The latest research has found that deer antler has significant effects on treating osteoarthritis, repairing cartilage defects, and inhibiting inflammation. Based on this, this article reviewed the pathogenesis of arthritis and the shortcomings of treatment methods, proposed that antler is an ideal model for repairing cartilage damage, and deeply discussed the repairing effect of antler and its derivatives on arthritis, emphasizing their dual effects in cartilage repair and inflammation inhibition, laying the foundation for the future application of deer antler and its derivatives in arthritis treatment.
AN Wei;LIU Weina;WANG Zhenlong;WANG Jinquan;WANG Xiumin;
Synthetic biology, one of the most groundbreaking scientific disciplines of the 21st century, is revolutionizing traditional industrial paradigms across pharmaceuticals, food, energy, and materials through its interdisciplinary integration of genetic engineering, protein engineering, and metabolic engineering. Concurrently, evolving socioeconomic conditions and lifestyle changes have led to growing popularity of companion animals–particularly cats and dogs–in urban households, owing to their demonstrated psychological benefits, including anxiety relief and stress reduction. This trend has spurred the development of specialized markets including pet food and nutrition, healthcare, and genetic breeding, which are emerging as significant drivers of global economic growth. Notably, China still exhibits significant gaps in fundamental research and technological applications in this field compared to international advanced levels. Thus, this review systematically summarizes the synthetic biology applications in the pet industry, highlighting breakthrough achievements in pet nutrition, medical health, and genetic disease prevention. These findings aim to provide theoretical foundations and technological pathways for scientific innovation and sustainable development of pet industry in China.
LIU Shibo;WANG Nan;REN Xiaoxia;MEI Hao;DUAN Yueqiang;CAI Yanan;
To establish an indirect ELISA method with good specificity and sensitivity for the detection of Chlamydia psittaci. The recombinant plasmid PfastBac1-dx was constructed according to the sequence of Cps ompA gene, and the target protein was expressed by bac to bac baculovirus expression system and purified by His nickel column. After identification by Western blot, the purified MOMP protein was used as detection antigen for coating. An indirect ELISA method was successfully established. The critical value, specificity and sensitivity of the method were further verified by systematically optimizing the key reaction conditions such as coating condition, blocking solution type, blocking time and serum incubation time. Western blot analysis showed that MOMP protein could specifically bind to Cps positive serum. The optimized ELISA reaction conditions were as follows: 10μg/mL antigen was coated at 4℃ for 12 h, 1% gelatin was blocked at 4℃ for 12 h, diluted serum was incubated at 37℃ for 2 h, and 1∶0.1ug/ml G-protein secondary antibody was incubated at 37℃ for 2 h. The sensitivity of this method is high, and the reaction is still obvious when the dilution of Cps positive serum is 1∶800. Compared with IHA kit, the total coincidence rate was 85.07%. This study established an indirect ELISA method with Cps MOMP protein as the detection antigen, which can simultaneously detect cattle and sheep Cps. The method has good sensitivity and specificity, and can be used for the serological detection of Cps, which provides technical support for the quality control of Chlamydia psittaci vaccine.
ZHANG Xinyu;LI Ke;LI Min;WANG Guiqin;KONG Yidi;
In the current context of the "ban on antibiotic resistance" in feed, exploring new feed additives to solve animal health problems has become a hot topic in the industry. Succinic acid, as a metabolite of the tricarboxylic acid cycle (TCA), is indispensable in biological organisms and plays a certain role in regulating animal energy metabolism, immune system, intestinal health, and antioxidation. It is a promising additive substance. With the deepening exploration of the mechanism of action of succinic acid, the exploration of its application in animal production is also gradually increasing. This article summarizes the physiological functions of succinic acid and its recent applications in animal production, aiming to provide scientific basis for future in-depth research on succinic acid and provide reference for its development and application in animal production.